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. 2020 Dec 4;11:588543. doi: 10.3389/fimmu.2020.588543

Figure 4.

Figure 4

ABT 317 reduces immune cell infiltration and T-cell proliferation in islets. (A, B): The percentage of CD45+ cells in islets from 13-week-old NOD mice treated with vehicle or 3 mg/kg or 10 mg/kg ABT 317 for 3 weeks. (A): Representative plots showing gating for CD45+ and CD45- islet cells. C: MFI of MHC class I expression on the β cell surface from NOD mice treated with vehicle or 3 or 10 mg/kg ABT 317 for 3 weeks. Data for (A–C) are n = 9 mice combined from two experiments (four to five mice per group in each experiment). (D–F): CD45+CD3+ live T cells in islets from 13-week-old NOD mice treated with vehicle or 3 or 10 mg/kg ABT 317 for 3 weeks. (D, E): Insulitis in pancreas harvested from 10 mg/kg ABT 317 (n = 3) or vehicle (n = 8) treated 150-day old female NOD mice. (D) Representative H&E stained sections, magnification 100×, scale bar 100 µm. (E) Pooled insulitis scores. P < 0.0001, two-way ANOVA. (F): Representative flow cytometry dot plots. (G, H): The numbers of CD8+ T cells (G) and CD4+ T cells (H). Data are n = 13 mice combined from three experiments (four or five mice per group in each experiment). Data are shown as the mean ± SEM. Statistical significance: *P < 0.05, One-way ANOVA with multiple comparisons. (I–L): The percentage of BrdU positive CD45+CD3+ T cells in islets from 13-week-old NOD mice treated with vehicle or 10 mg/kg ABT 317 for 3 weeks. (I, K): Representative flow cytometry dot plots. (J, K): The percentage of BrdU positive CD8+ T cells (J) and the percentage of BrdU positive CD4+ T cells (L). Data are n = 8–9 mice with the mean ± SEM for two independent experiments. Statistical significance: *P < 0.05, ***P = 0.0002, Student t test.