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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Environ Pollut. 2020 Oct 10;268(Pt A):115601. doi: 10.1016/j.envpol.2020.115601

Figure 6. IAA exposure disrupted MII spindle formation and chromosome alignment.

Figure 6.

(A) Representative images of spindle morphology and chromosome alignment in control and IAA-treated MII oocytes. α-tubulin, green; chromosomes/DNA, blue. Scale bar, 20 μm. Arrows highlight the misaligned chromosomes. (B) The percentage of oocytes with abnormal spindles in the control and 5 μM IAA-treated groups. (C) The proportion of oocytes with misaligned chromosomes in the control and 5 μM IAA-treated groups. A total of 54 MII oocytes in the control and 54 in the 5 μM IAA-treated group were used for analysis. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01, compared with control.