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. Author manuscript; available in PMC: 2021 Feb 28.
Published in final edited form as: Nature. 2020 Aug 31;588(7838):459–465. doi: 10.1038/s41586-020-2709-7

Extended Data Figure 6 |. Grn−/− microglial conditioned media (MCM)-induced cell death in Grn+/+ and Grn−/− cortical neurons and GABAergic neurons.

Extended Data Figure 6 |

a. Representative confocal images of Grn+/+ and Grn−/− cortical neurons treated with control media, Grn+/+ MCM or Grn−/− MCM (100μg/ml) overnight. Immunofluorescent stains are performed using antibodies for MAP2 (green) and cleaved caspase 3 (red). Nuclei are highlighted using DAPI. b. Representative confocal microscopic images of GE-derived Grn+/+ and Grn−/− GABAergic interneurons treated with control media, Grn+/+ MCM or Grn−/− MCM (100μg/ml) overnight. Immunofluorescent stains are performed using antibodies for GAD67 (green) and cleaved caspase 3 (red). Nuclei are highlighted using DAPI.