Figure 3. Biophysical characterization of individual lipid binding events to K_2P4.1b.

A) Representative mass spectra of K_2P4.1b with 20 μM POPE in C₁₀E₅ and 10 mM spermidine collected at room temperature. Deconvolution of the mass spectrum is shown to the right. B) Binding constants for PO-type lipids binding to K_2P4.1b. C) K_D values for K_2P4.1b binding PA and PE with different acyl chains and PEth. The different linkage of PL lipids impacts their bind affinity to K_2P4.1b. D) Binding constants for binding PI and PIPs to K_2P4.1b. E) K_D values for LyPE and LyPA lipids binding to K_2P4.1b. Lipid abbreviations are listed in Supplementary Table 1. Data are presented as the mean ± standard deviation (n = 3) for three independent experiments (white dots).