Extended Data Figure 3. Determination of equilibrium dissociation constants (K_D) for lipids binding K_2P4.1a.

Shown are representative native mass for K_2P4.1a in the presence of 20 μM of lipid. Plots of mole fraction (right panel) for apo K_2P4.1a and bound to different number of lipids determined from a titration series (dots with error bars to represent the three measurements) and resulting fit from a sequential lipid-binding model (solid lines). Lipid abbreviations are provided in Supplementary Data Table 1.