Figure 3.

Effect of gigantol on the JNK and cPLA2 activation in livers induced by CCl₄. (a) The protein expression of p-JNK, JNK, p-cPLA2 and cPLA2 in mice with or without gigantol pretreatment (40 mg kg⁻¹ d⁻¹, po, for 7 days) at 2 h or 16 h post CCl₄ injection. The representative western blot bands are shown, n = 3 western experiments. The ratio of p-JNK/JNK and p-cPLA2/cPLA2 is quantified on the right. (b) The mRNA expression of PLA2 in the mouse livers were measured by real-time PCR. (c) The concentration of AA in the livers was measured by UPLC/MS/MS. The above results are shown as the mean ± SD, n = 6 mice in each group. ^###P < 0.001, ^##P < 0.05 versus the vehicle group; ***P < 0.001 versus the CCl₄ group; ns means non-significance. Full-length blots are presented in Supplementary Figure S5.