Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 17;10:22190. doi: 10.1038/s41598-020-79102-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of S. cerevisiae and Selemax on the proportion of Tregs and tolerogenic DC in mesenteric lymph nodes. EAE mice received 14 oral doses of S. cerevisiae or Selemax. Total number of leukocytes present in mesenteric lymph nodes (MLN) per gram of tissue (a). Total cells from MLN were collected and analyzed by flow cytometry to establish the proportion of T helper cells (Th - CD3⁺CD4⁺) (b) and regulatory T cells (Treg - CD25⁺Foxp3⁺) (c). The proportion of T subsets was used to calculate the ratio between Treg and Th cells (d). Representative gating strategy to assess dendritic cell (DC - CD11c⁺MHCII^High) subsets in inguinal lymph nodes (e) and the mean fluorescence intensity (MFI) of PD-L1 in tolerogenic DC (CD11b^-CD103⁺) subset (f). The proportion of CD11b⁺CD103^- (g), CD11b⁺CD103⁺ (h) and CD11b^-CD103⁺ (i) DC subsets in total DC. The MFI of PD-L1 was evaluated in CD103⁺CD11b^- DC subset (j). Statistical analysis was performed by one-way ANOVA and subsequent Tukey’s test. All data were expressed as the mean ± SD and statistical differences were represented by *p < 0.05 and **p < 0.01. Two independent experiments were combined, n = 6–8 mice/group. The graphs were created using GraphPad Prism v.8.0.2, flow cytometry images were created using FlowJo 10.7.1 and image was modified with Adobe Photoshop v.22.0.0.