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. 2020 Dec 17;10:22190. doi: 10.1038/s41598-020-79102-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of S. cerevisiae and Selemax on microbiota composition. EAE mice received 14 oral doses of S. cerevisiae or Selemax. Principal Coordinates Analysis (PCoA) of unweighted UniFrac distances of 16 S rRNA genes (a). Absolute abundance (b), richness (c), evenness (d), Shannon diversity index (e) and Faith’s phylogenetic index (f) of fecal bacterial OTUs. Relative abundance at phylum (g), family (h) and genus (i) level. Statistical analysis was performed by one-way ANOVA and subsequent Tukey’s test (b–f) and two-way ANOVA and subsequent Tukey’s test (g–i). All data were expressed as the mean ± SD and statistical differences were represented by *p < 0.05 and **p < 0.01; ^# p < 0.05 between EAE and EAE/S. cerevisiae; ^&p < 0.05 between EAE and EAE/Selemax. One experiment representative of two independent experiments (a) and two independent experiments were combined (b–i), n = 4–9 mice/group. PCoA was created using QIIME2 v.2020.2 (https://view.qiime2.org/), the graphs were created using GraphPad Prism v.8.0.2 and image was modified with Adobe Photoshop v.22.0.0.