Table 1.
ANOVA results evaluating the effects of sex, age, and capture location on measures of diet, body condition, and microbiome alpha diversity.
| Mean Value | Sex | Age | Location | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Urban | Rural | F | df | p | F | df | p | F | df | P | |
| Mass (kg) | 10.30 | 10.85 | 23.48 | 1 | < 0.001 | 48.90 | 1 | < 0.001 | 0.34 | 1 | 0.725 |
| Body size (cm) | 84.87 | 87.31 | 8.08 | 1 | 0.006 | 31.94 | 1 | < 0.001 | 1.44 | 1 | 0.233 |
| Girth (cm) | 45.73 | 46.87 | 11.29 | 1 | < 0.001 | 25.70 | 1 | < 0.001 | 0.17 | 1 | 0.684 |
| Age (years) | 1.82 | 2.64 | 5.70 | 1 | 0.019 | – | – | – | 3.18 | 1 | 0.078 |
| Spleen size (g/kg) | 2.34 | 1.70 | 1.20 | 1 | 0.276 | 2.51 | 1 | 0.117 | 28.01 | 1 | < 0.001 |
| KFI | 0.32 | 0.57 | 2.06 | 1 | 0.150 | 1.68 | 1 | 0.198 | 15.14 | 1 | < 0.001 |
| Health index | − 0.02 | 0.01 | 13.27 | 1 | < 0.001 | 40.93 | 1 | < 0.001 | 0.01 | 1 | 0.316 |
| δ13C (‰) | − 21.48 | − 22.90 | 0.01 | 1 | 0.926 | 1.38 | 1 | 0.243 | 54.11 | 1 | < 0.001 |
| δ15N (‰) | 8.69 | 8.96 | 1.53 | 1 | 0.220 | 1.98 | 1 | 0.163 | 2.24 | 1 | 0.138 |
| Vol. anthro food (ml) | 34.42 | 24.61 | 0.07 | 1 | 0.793 | 3.21 | 1 | 0.076 | 0.24 | 1 | 0.627 |
| Vol. prey (ml) | 59.97 | 211.58 | 1.06 | 1 | 0.306 | 1.21 | 1 | 0.274 | 5.75 | 1 | 0.018 |
| ASV richness | 333.40 | 294.28 | 0.12 | 1 | 0.738 | 0.90 | 1 | 0.344 | 1.93 | 1 | 0.060 |
| Shannon index | 3.11 | 2.68 | 0.48 | 1 | 0.492 | 0.17 | 1 | 0.678 | 5.71 | 1 | 0.019 |
| Faith's PD | 44.27 | 31.34 | 0.37 | 1 | 0.546 | 1.25 | 1 | 0.270 | 38.68 | 1 | < 0.001 |
| NTI | 6.47 | 4.40 | 1.89 | 1 | 0.173 | 1.37 | 1 | 0.245 | 33.11 | 1 | < 0.001 |
All variables were tested using a linear regression, with the exception of ASV richness, which was tested using a negative binomial regression. Stomach contents were log-transformed prior to testing to meet the assumptions of a normal distribution.
KFI kidney fat index, ASV amplicon sequence variant, PD phylogenetic diversity, NTI nearest taxon index.