Fig. 1. Immune checkpoint blockade and radiation therapy cure transplant but not primary sarcomas.

a Transplant tumor initiation by p53/MCA cell injection into the gastrocnemius. Mice were treated with anti (α)-PD-1 (red) or isotype control (blue) antibody and 0 (solid) or 20 (dashed) Gy when tumors reached >70 mm³. b Primary sarcoma initiation by intramuscular injection of Adeno-Cre and MCA. Treatment as in panel (a). c Mice with transplant sarcomas received either both isotype control antibodies with 0 Gy (solid blue, n = 10), αPD-1 and αCTLA-4 with 0 Gy (solid red, n = 11), both isotype control antibodies with 20 Gy (dashed blue, n = 10), or αPD-1 and αCTLA-4 with 20 Gy (dashed red, n = 10). d Mice with transplant sarcomas received either both isotype control antibodies with 0 Gy (solid blue, n = 22), αPD-1 and αCTLA-4 with 0 Gy (solid red, n = 22), both isotype control antibodies with 20 Gy (dashed blue, n = 20), or αPD-1 and αCTLA-4 with 20 Gy (dashed red, n = 18). Survival curves estimated using Kaplan–Meier method; pairwise significance determined by log-rank test and Bonferroni correction. *P < 0.05. Source data are provided as a Source data file.