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. 2020 Dec 17;10:22175. doi: 10.1038/s41598-020-79249-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of protein restriction on body weight. (A) Method of protein restriction (PR). Mice at 2 months old (preintervention) were divided into two groups: controls, fed a control diet for the following 10 months (353.6 kcal/100 g); and PR, fed a protein-restricted diet for the following 10 months (353.8 kcal/100 g). All mice were exposed to odors for c-fos experiments before fixation. (B) Body weights of control (left) and PR mice (right) at preintervention (2-month-old mice), 5 months (7-month-old mice), and 10 months (12-month-old mice) in each group (eight mice in each group). Data are shown as mean ± SD. (C) Percent body weight differences at preintervention, 5 months, and 10 months (eight mice in each group). At 5 and 10 months, a significant reduction in percent body weight change was observed in PR mice compared with control mice (*P < 0.05, **P < 0.01, Mann–Whitney test). Data are shown as mean ± SD.