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. 2020 Dec 17;10:22175. doi: 10.1038/s41598-020-79249-3

Figure 2.

Figure 2

Effects of 10 months of PR on age-related degenerative changes in the inner ear. (A) Schematic diagram of the organ of Corti. The closed black circle indicates a surviving inner hair cell (IHC), and the closed red circle indicates a surviving outer hair cell (OHC). (B) Hematoxylin and eosin-stained images at the apical turn of the organ of Corti in control and PR mice. The open circle represents the loss of hair cells. Scale bar 50 μm. (C) Survival rates of IHCs in control and PR mice. At basal and apical turns, no significant differences in IHCs were found between PR and control mice (Mann–Whitney test). (D) Survival rates of OHCs in control and PR mice. The OHC survival rate in the PR mice was significantly higher than that in the controls only at the apical turn (*P < 0.05, Mann–Whitney test). (E) Representative images of spiral ganglion cells (SGCs) at the apical turn in control and PR mice. Scale 50 μm. (F) Summary of the SGC density at the basal and apical turns. Significantly higher averaged SGC density in the PR mice compared with the controls was observed only at the apical turn (**P < 0.01, Mann–Whitney test).