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. 2020 Dec 17;10:22175. doi: 10.1038/s41598-020-79249-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PR induces OE injury in the dorsomedial area of the OE. (A–C) Photomicrographs of representative coronal sections of the olfactory epithelium (OE) in preintervention mice (A), control mice (B), and PR mice (C). The unilateral OE was divided into four areas: dorsomedial (DM), dorsolateral (DL), ventromedial (VM), and ventrolateral (VL). Left images, low magnification; middle (HE-staining) and right images (anti-OMP staining), high magnification. Scale 300 µm at low magnification, 50 µm at high magnification. (D,E) Numbers of OSNs (D) and numbers of OMP-positive cells (E) in each area (DM, DL, VM, and VL). Significant histological changes in each area were observed between preintervention and control mice, while a significant difference between control mice and PR mice was detected only in the DM area (***P < 0.001, Mann–Whitney test).