Figure 5.

PR results in the projection of axons from fewer OSNs of zone 1. (A) Representative glomeruli (upper, NQO1-positive OB; lower, NQO1-negative OB) stained with anti-OMP antibody (green) from control and PR mice. Each circled area corresponds to a glomerulus. Scale 50 µm. (B) Summary of the ratio of areas stained with OMP. The OMP-stained area in the NQO1-positive OB of PR mice was significantly lower than that in control mice (***P < 0.001, Mann–Whitney test). However, the OMP-stained area did not differ according to condition in the NQO1-negative OB. (C) Representative coronal sections stained with anti-c-fos antibody in the NQO1-positive and NQO1-negative OB of control and PR mice. Aldehydes, lactones, and esters were selected as stimulus odorants to induce c-fos immunoreactivity in the OB neurons. Each circled area corresponds to a glomerulus. Scale 50 µm. (D) Comparison of the number of c-fos-positive cells between NQO1-positive and NQO1-negative OB in control and PR mice. The number of c-fos-positive cells (per mm²) in the NQO1-positive OB of PR mice was significantly less than that in control mice (***P < 0.001, Mann–Whitney test), whereas in the NQO1-negative OB of PR mice, no significant difference was detected.