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. 2020 Dec 17;10:22204. doi: 10.1038/s41598-020-78903-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The protein levels of nNOS, TrkB, Bcl-2, Bax, and caspase-3 activation in hippocampal neurons. (a) nNOS, TrkB, Bcl-2, and Bax protein levels in hippocampus neurons were measured by western blotting, then intensity was calculated using β-actin as an internal control to normalize protein loading (b–e). (f) caspase-3 activation. nNOS neuronal nitric oxide synthase, TrkB tropomyosin-related kinase B, Bcl-2 B-cell lymphoma-2, Bax Bcl-2-associated X. n = 3, represents three biological replicates, each performed in triplicate. *P < 0.05, **P < 0.01, based on one-way analysis of variance (ANOVA) followed by least significant difference (LSD) post hoc test for multiple comparisons.