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. 2020 Dec 17;10:22258. doi: 10.1038/s41598-020-79415-7

Table 3.

Sperm kinematic data of CASA analysis after 10 min at 38 °C of boar spermatozoa in sixteen pooled ejaculates (4 × 4 ejaculates) from 16 mature Pietrain boars in control and MEDI NOVA bags.

Characteristics Storage time (h) Control Bags P-value
Progressive motility (%) 24 84.8 ± 2.1 80.3 ± 0.4 0.021
Distance curved line (µm) 69.3 ± 5.8 68.2 ± 0.7 0.692
Distance straight line (µm) 22.7 ± 0.9 21.1 ± 1.3 0.016
Distance average path (µm) 34.4 ± 2.1 33.3 ± 1.2 0.430
Velocity curved line (µm/s) 153.7 ± 11.5 150.4 ± 3.0 0.581
Velocity straight line (µm/s) 54.7 ± 2.1 51.5 ± 2.0 0.021
Velocity average path (µm/s) 78.4 ± 2.4 76.0 ± 1.6 0.247
Beat cross frequency (Hz) 25.8 ± 1.0 24.1 ± 0.7 0.056
Amplitude of lateral head displacement (µm) 1.36 ± 0.11 1.34 ± 0.05 0.689
Progressive motility (%) 48 82.5 ± 3.1 79.7 ± 3.2 0.006
Distance curved line (µm) 69.4 ± 2.2 62.8 ± 2.4 0.018
Distance straight line (µm) 22.0 ± 2.4 22.1 ± 1.6 0.917
Distance average path (µm) 33.8 ± 1.6 31.6 ± 1.4 0.062
Velocity curved line (µm/s) 155.4 ± 5.3 140.4 ± 5.2 0.017
Velocity straight line (µm/s) 54.9 ± 3.6 54.5 ± 1.9 0.738
Velocity average path (µm/s) 78.8 ± 2.4 73.8 ± 1.9 0.035
Beat cross frequency (Hz) 24.3 ± 1.3 23.1 ± 1.0 0.053
Amplitude of lateral head displacement (µm) 1.38 ± 0.07 1.25 ± 0.06 0.07
Progressive motility (%) 96 81.0 ± 4.1 78.6 ± 3.6 0.004
Distance curved line (µm) 64.7 ± 1.2 60.6 ± 2.6 0.093
Distance straight line (µm) 23.4 ± 2.5 22.4 ± 1.5 0.300
Distance average path (µm) 32.6 ± 1.1 30.8 ± 1.7 0.070
Velocity curved line (µm/s) 145.8 ± 5.4 138.6 ± 5.6 0.135
Velocity straight line (µm/s) 57.7 ± 3.2 56.8 ± 2.7 0.464
Velocity average path (µm/s) 76.5 ± 2.4 73.9 ± 2.8 0.017
Beat cross frequency (Hz) 23.7 ± 1.5 22.0 ± 1.3 0.013
Amplitude of lateral head displacement (µm) 1.28 ± 0.07 1.21 ± 0.05 0.164
Progressive motility (%) 120 77.5 ± 5.3 75.8 ± 3.9 0.383
Distance curved line (µm) 59.5 ± 1.6 54.4 ± 4.6 0.063
Distance straight line (µm) 22.4 ± 3.0 22.3 ± 1.9 0.886
Distance average path (µm) 30.5 ± 1.8 28.7 ± 2.3 0.139
Velocity curved line (µm/s) 135.8 ± 5.2 124.2 ± 10.8 0.036
Velocity straight line (µm/s) 56.1 ± 5.7 54.1 ± 3.8 0.127
Velocity average path (µm/s) 72.6 ± 3.9 67.4 ± 4.4 0.016
Beat cross frequency (Hz) 22.4 ± 1.6 20.4 ± 0.5 0.074
Amplitude of lateral head displacement (µm) 1.17 ± 0.05 1.12 ± 0.13 0.376
Progressive motility (%) 168 70.0 ± 2.7 47.0 ± 10.1 0.018
Distance curved line (µm) 52.7 ± 2.7 30.7 ± 7.1 0.006
Distance straight line (µm) 20.4 ± 2.2 12.4 ± 1.8 0.025
Distance average path (µm) 26.1 ± 1.3 15.3 ± 2.8 0.010
Velocity curved line (µm/s) 123.8 ± 9.3 73.5 ± 16.8 0.002
Velocity straight line (µm/s) 53.1 ± 4.9 33.4 ± 3.8 0.011
Velocity average path (µm/s) 65.1 ± 4.7 39.6 ± 6.2 0.004
Beat cross frequency (Hz) 18.4 ± 0.8 11.1 ± 2.7 0.022
Amplitude of lateral head displacement (µm) 1.11 ± 0.09 0.74 ± 0.17 0.009

Significant differences are marked with italics.