Figure 5.

Measured kinetic data of Ni(II) binding obtained at high FliC-NikR_C coverage on a PCP-LIP WAVEchip (red curves). The shown measurement curves were adjusted and represent Ch1-Ch2 reference corrected data. The data were fitted using the heterogeneous ligand kinetic model (black curves). The spikes at association start and dissociation start annotations were originated from solution exchange effect. The inset scheme illustrates the binding of Ni to the FliC-NikR_C tetramers which were immobilized on the quasi-planar lipophilic coating of the PCP-LIP chip. While the solid circles with dark green color represent Ni(II) ions bound to the HA sites, the light green circles represent Ni(II) ions bound to the LA sites. Kinetic data determined from the model fit are shown in the right table. Here, subscript index 1 and 2 correspond to the HA and LA sites, respectively, R_max is the maximal response value, k_a and k_d are the association and dissociation rate constants as well as K_d is the dissociation constant.