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. 2020 Dec 17;10:22253. doi: 10.1038/s41598-020-79226-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Measured kinetic data of Ni(II) binding obtained at high FliC-NikR_C coverage on a PCH WAVEchip (red curves). The shown measurement curves were adjusted and represent Ch1-Ch2 reference corrected data. The data were fitted using the heterogeneous ligand kinetic model (black curves). The inset scheme illustrates the binding of Ni to the FliC-NikR_C monomers which were immobilized on the thick hydrogel coating of the PCH chip. The light green circles represent Ni(II) ions bound to LA sites.