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Fluorescence microscopic investigation of Live/Dead stained biofilm allows bacterial coverage visualization and differentiation between live (green) and dead (red) bacteria. After 48 h, non-polished samples presented significantly higher amounts of biofilms than polished samples. While water negative controls appeared densely covered with live bacteria, samples rinsed with chlorhexidine 0.2% and hydroxyapatite nanoparticles presented less biofilm formation.
