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Activity measured on different parts of the cassette immediately after synthesis on ML eazy at 98 °C (n = 3)
| load [μg] | product vial [%] | CM cartridge [%] | waste [%] | SCX cartridge [%] | reaction vial [%] |
|---|---|---|---|---|---|
| 10 | 36.1 ± 15.5 | 59.7 ± 15.6 | 0.0 ± 0.0 | 0.4 ± 0.0 | 3.7 ± 0.2 |
| 20 | 75.3 ± 18.2 | 19.3 ± 14.2 | 0.2 ± 0.2 | 0.4 ± 0.1 | 4.8 ± 4.2 |
| 30 | 88.7 ± 10.1 | 9.2 ± 9.7 | 0.0 ± 0.0 | 0.5 ± 0.1 | 1.6 ± 0.5 |
| 40 | 85.5 ± 11.9 | 11.0 ± 9.6 | 0.1 ± 0.1 | 0.5 ± 0.2 | 2.9 ± 3.0 |
| 50 | 89.7 ± 6.7 | 6.1 ± 5.5 | 0.0 ± 0.0 | 0.5 ± 0.1 | 3.7 ± 1.4 |
