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. 2020 Dec 4;13:232. doi: 10.3389/fnmol.2020.603409

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Copyright © 2020 Szczygieł, Danielsen, Melin, Rosenkranz, Pankratova, Ericsson, Agerman, Kokaia and Woldbye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neuronal tropism in the dorsal hippocampal DG and CA3 of CG01-treated rat as evidenced by NPY-immunoreactivity being co-expressed with NeuN-immunoreactivity as revealed by fluorescent (A–C) and confocal microscopy (D–F). (A) Fluorescent microscopy overview images of NPY/NeuN co-staining (merge), NPY- and NeuN-immunoreactivity, with high-magnification images of hatched areas in panel (A), showing hilus of the DG (hDG; B) and CA3 (B,C). (D) Confocal microscopy overview image of NPY/NeuN co-staining, with high-magnification images of hatched areas in panel (D), showing co-labeling in fibers and interneuron (white arrow) in hDG (E) and fibers in pyramidal layer of CA3 (pyrCA3; F). Scale bars: 500 μm (A), 100 μm (B–D), and 30 μm (E,F).