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. 2020 Dec 4;10:573127. doi: 10.3389/fonc.2020.573127

Figure 1.

Figure 1

RPM8 expression activates basal autophagy level. (A, B) HeLa cells were incubated with 10 M CQ for the indicated times. The cell lysates were subjected to western blot (WB) analysis using indicated antibodies (N = 3). (C, D) Construct for TRPM8 expression was transfected into HeLa cells for 48 h; the cells were used for WB analysis using indicated antibodies (N = 3). (E, F) Construct for ptfLC3 expression was cotransfected with the TRPM8 or control vector into HeLa cells for 48 h, and accumulation of yellow and red puncta were observed by fluorescence microscopy; representative confocal images are shown (scale bar, 20 μm). (G, H) WB analysis was performed in the lysates of HeLa cells transfected with TRPM8-specific siRNA to decrease TRPM8 expression (N = 3). (I, J) Yellow and red puncta in HeLa cells transfected with TRPM8 siRNA. N represents the number of replicate experiments. Ctrl, control; CQ, chloroquine; LC3, microtubule-associated protein 1 light chain 3. *P < 0.05; NS, not significant.