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. 2020 Dec 4;10:573127. doi: 10.3389/fonc.2020.573127

Figure 2.

Figure 2

Mechanism of the stimulatory effect of TRPM8 channel function on basal autophagy. (A, B) HeLa cells were incubated with menthol (10 μM) for 48 h. The cells were lysed and assayed by western blot using indicated antibodies (N = 3). (C, D) WB analysis of HeLa cell lysates after treatment with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h (N = 3). (E, F) HEK293 cells were cotransfected with EGFP and wild type TRPM8, mutant, or a control vector. After 48 h of transfection, EGFP-positive cells were selected for recording of TRPM8 channel-mediated current. The number of cells used for current recordings: Vector (6); WT: (10); V976W (12). (G, H) HeLa cells were transfected with the constructs for the expression of wild type or mutant TRPM8. After 48 h of transfection, cells were lysed and subjected to WB analysis using indicated antibodies (N = 3). N represents the number of replicate experiments. EGFP, enhanced green fluorescent protein; Ctrl, control; *P < 0.05; NS, not significant.