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. 2020 Dec 4;10:573127. doi: 10.3389/fonc.2020.573127

Figure 3.

Figure 3

Upregulation of autophagy by TRPM8 in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM icilin, 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P < 0.05; NS, not significant.