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. 2020 Dec 4;10:584477. doi: 10.3389/fonc.2020.584477

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Copyright © 2020 Tymoszuk, Nairz, Brigo, Petzer, Heeke, Kircher, Hermann-Kleiter, Klepsch, Theurl, Weiss and Pfeifhofer-Obermair

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of intravenous iron on CD4⁺ TILs (A), regulatory T cells (B) and Th1/Treg or Tc1/Treg ratios (C, D) in immunotherapy and chemoimmunotherapy of mouse mammary carcinomas. Ratios Th1/Treg (C) and Tc1/Treg (D) were calculated. Representative plots are shown (mean ± SEM). Statistical significance was determined by 2-way ANOVA. The results of Tuckey post-hoc-test are presented in the plots: ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. untreated n = 24, untreated + iron n = 3, IL-2 + doxorubicin n = 14, IL-2 + doxorubicin + iron n = 17, aPD-L1 n = 13, aPD-L1 + iron n = 12. The results of ANOVA are presented in Materials and Methods/Specific statistical data analyzed in main figures.