Figure 7.

Iron administration to splenocytes leads to oxidative stress and increased production of mitochondrial reactive oxygen species (ROS). Splenocytes were isolated from tumor-naive C57Bl/6N female mice and cultured in 96 well plates coated with anti-CD3. Fe₂(SO₄)₃ and holo-transferrin were added as NTBI and TBI, the inhibitor of oxidative phosphorylation rotenone was used as a positive control for ROS formation. After 24h DCFDA⁺ and MitoSox⁺ CD8⁺ T cells (A) and CD4⁺ T cells (B) were analysed by flow cytometry. DCFDA is defined as indicator for cytoplasmic ROS, MitoSox for mitochondrial ROS. Statistical significance was determined by Student`s t-test. Representative flow cytometry results and summary plots are shown (mean±SEM; n = 3).