Figure 3.

Knockdown of FAM83A expression inhibits cervical cancer cell proliferation in vitro and in vivo. (A) qRT-PCR and western blot analysis were performed to detect the expression of FAM83A in cervical cancer cell lines, normal human cervical epithelial cells (HCerEpiC), two low metastatic cervical cancer cell lines (HeLa and SiHa) and one highly metastatic cell line (CaSki). (B) qRT-PCR and immunoblot analysis confirmed that the FAM83A expression levels were significantly knocked down in CaSki cells. (C) FCM analysis of the effect of FAM83A knockdown on cell cycle distribution. (D) A CCK-8 assay was performed to detect cell proliferation of the control or siFAM83A-transfected CaSki cells. (E) Knockdown of FAM83A inhibits CaSki cell tumor growth in vivo. (F) Ki67 immunostaining of tumor samples from the FAM83A knockdown and control CaSki groups. Error bars represent the means of three independent experiments. ^*P < 0.05, ^**P < 0.01.