Fig. 4.

Effect of MEG3 over-expression on the growth of brain xenografts of GFP expressing GSC #1. (A) Kaplan–Meier analysis of mice with brain grafts of GSCs (left). Mice grafted with MEG3 GSCs showed weight loss (>20% of initial weight) or neurological signs later than those grafted with control GSCs (n = 12; P = 0.0133, log rank test). Coronal sections of brain across the grafting site in a control and MEG3 mouse (right). (B) The density of tumor cells spreading in the striatum, amygdala, anterior commissure, and optic chiasm is highly reduced in xenografts of MEG3 overexpressing cells. (C) Graphs showing results of tumor cell counts in the brain regions analyzed; **P < 0.01; ***P < 0.001.