Table 3.
Spontaneous chromosomal aberrations per 60 cells
| Cell lines | Days after conditional inactivation | Treatment for conditional inactivation | Chromatid | Isochromatid | Exchanged | Total aberration (±SE)† |
|---|---|---|---|---|---|---|
| DT40 | Wild-type cells (Hoa et al. 2015) | |||||
| No | 1.1 | 0 | 0 | 1.1 ± 1.0 | ||
| MRE11−/H129N cells | ||||||
| No | 2.8 | 1.9 | 0.9 | 5.6 ± 2.3 | ||
| EXO1−/− DT40 cells | ||||||
| No | 1.1 | 0 | 0 | 1.1 ± 1.0 | ||
| DNA2−/− cells (Hoa et al. 2015) | ||||||
| Day 0 | No | 1.2 | 0.0 | 0.0 | 1.2 ± 1.0 | |
| Day 1 | Doxycycline | 2.4 | 1.2 | 0.0 | 3.6 ± 1.9 | |
| Day 2 | 6.0 | 1.2 | 1.2 | 8.4 ± 2.9 | ||
| Day 3 | 19.2 | 18 | 2.4 | 39.6 ± 6.3 | ||
| DNA2−/D245A cells (Hoa et al. 2015) | ||||||
| Day 0 | No | 1.2 | 1.2 | 0.0 | 2.4 ± 1.5 | |
| Day 1 | Doxycycline | 3.8 | 0.9 | 0.0 | 4.8 ± 1.6 | |
| Day 2 | 4.8 | 1.2 | 0.0 | 6.0 ± 2.4 | ||
| Day 3 | 20.4 | 14.4 | 1.2 | 36.0 ± 6.0 | ||
| DNA2−/K623E cells | ||||||
| Day 0 | No | 1.2 | 0.0 | 0.0 | 1.2 ± 1.0 | |
| Day 1 | Doxycycline | 4.2 | 1.5 | 0.3 | 6.0 ± 1.6 | |
| Day 2 | 3.6 | 3.6 | 0.0 | 7.2 ± 2.7 | ||
| Day 3 | 13.2 | 18.0 | 3.6 | 34.8 ± 5.9 | ||
| DNA2−/D245A/EX01−/− cells | ||||||
| Day 0 | No | 1.0 | 1.0 | 0.0 | 2.0 ± 1.4 | |
| Day 1 | Doxycycline | 3.6 | 0.9 | 0.0 | 4.5 ± 1.6 | |
| Day 2 | 4.8 | 2.4 | 1.2 | 8.4 ± 2.6 | ||
| Day 3 | 15.0 | 16.9 | 3.8 | 35.7 ± 5.9 | ||
| TK6 | Wild-type cells | |||||
| No | 1.5 | 0 | 0 | 1.5 ± 1.2 | ||
| MRE11−/− cells | ||||||
| Day 0 | No | 2.1 | 0.0 | 0.0 | 2.1 ± 1.3 | |
| Day 4 | Tamoxifen | 1.8 | 0.0 | 0.0 | 1.8 ± 1.1 | |
| Day 5 | 2.3 | 3.0 | 3.8 | 9.0 ± 2.2 | ||
| Day 6 | 7.6 | 13.1 | 21.8 | 42.5 ± 5.7 | ||
| MRE11−/Hl29N cells | ||||||
| Day 0 | No | 1.5 | 0.0 | 0.6 | 2.1 ± 1.2 | |
| Day 4 | Tamoxifen | 1.7 | 0.0 | 0.3 | 2.0 ± 1.3 | |
| Day 5 | 5.9 | 4.6 | 1.4 | 11.8 ± 3.1 | ||
| Day 6 | 18.3 | 4.7 | 1.8 | 24.8 ± 4.5 | ||
| CtIPmAID/mAID cells | ||||||
| Day 0 | No | 5 | 8 | 0.0 | 13 ± 3.5 | |
| Day 1 | Auxin | 22 | 27 | 2 | 51 ± 5.6 | |
| CtIPWT/WT cells | ||||||
| No | 2.1 | 0.0 | 0.6 | 3.0 ± 1.6 | ||
| CtIPND/ND cells | ||||||
| No | 2.1 | 0.0 | 0.6 | 3.0 ± 1.6 |
†
Data are presented as the number of aberrations per 60 cells. The actual number of cells analyzed was 60 for each genotype. Defining the number of cells analyzed and total chromosomal aberrations as N and x, respectively, we calculated the number of total aberrations per cell ± SE as , based on the Poisson distribution of spontaneous chromosomal aberrations previously observed (Sonoda et al. 1998). ‘Days’ indicates the duration of treatment with either doxycycline, tamoxifen or auxin.