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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Leukoc Biol. 2019 Jul 1;106(5):1139–1151. doi: 10.1002/JLB.3A0119-025RRR

FIGURE 3. Effect of RhoA deficiency on Treg cells.

FIGURE 3

(A-C) Total thymocytes and splenocytes were prepared from conditional RhoA-deficient mice and their WT littermates. Cells were processed for Treg staining. Percentages of natural Treg (nTreg, Foxp3+) in thymic single CD4+(SP4) and splenic CD4+ cells are shown in representative dot plots (A) and summarized in bar graphs (B). The absolute numbers of nTreg are also shown (C) (mean + SD, n = 5). (D) Naive CD4+ T cells were differentiated under Treg polarizing condition with TGF-β1 for 4 days and restimulated with PMA plus ionomycin. Percentages of Foxp3+ cells are shown in representative dot plots. Data are representative of 2 independent experiments with similar results. **P < 0.01 compared to WT mice