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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Leukoc Biol. 2019 Jul 1;106(5):1139–1151. doi: 10.1002/JLB.3A0119-025RRR

FIGURE 8. Y16 treatment downregulates pulmonary Th17 and Th2 cells from HDM-inoculated mice.

FIGURE 8

Allergic airway inflammation was induced by HDM and treated with Y16 as described in Fig. 7A. Mice were sacrificed 24 h after the last HDM challenge and the pulmonary infiltrating cells were isolated. The cells were restimulated in vitro with PMA plus ionomycin for 4 h with BD GolgiPlug in the last 3 h for FACS stainings. Percentages of cytokine or Foxp3 positive cells gated on CD4+ T cells are shown in representative dot plots (A). The isotype controls from pooled cells are also shown (A, Right). The numbers of total pulmonary infiltrating cells and CD4+ T cells (B), cytokine or Foxp3 positive cells gated on CD4+ cells (C) are summarized in bar graphs (means + SE, n = 3-6). *P < 0.05 versus HDM-challenged vehicle-treated groups