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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: Plant Cell Environ. 2019 Jul 17;42(9):2664–2680. doi: 10.1111/pce.13569

FIGURE 5.

FIGURE 5

Silencing of ShARPC3 in tomato reduced defence responses and increased On-Lz infection. (a) Histological observation of hypersensitive cell death in CK (TRV2) and TRV2:ShARPC3 tomato leaves inoculated with On-Lz by microscopy. Blue (trypan) staining indicates hypersensitive cell death. (b) Histological observation of H2O2 accumulation in CK and TRV2:ShARPC3 tomato leaves inoculated with On-Lz by microscopy. (c) HR production rate of tomato leaves carrying TRV2 (CK) or TRV2:ShARPC3 at 6, 18, 24, 48, and 72 hpi, respectively. (d) H2O2 production in CK or TRV2:ShARPC3 tomato leaves at 6, 18, 24, 48, and 72 hpi, respectively. Agt, appressorium germ tube; App, appressorium; Co, conidium; Ha, haustorium; HR, hypersensitive response; Pa, papilla; Sa, secondary appressorium; Sh, secondary hyphae. Bar, 50 μm. Error bars represent the variations among three independent replicates. The asterisk (*) indicates statistically significant differences between CK (TRV2) and TRV2:ShARPC3 plants (P < .05)