SA content and relative mRNA transcript levels of PRs genes are reduced in On-Lz-inoculated ShARPC3-silenced plants. (a) Changes in SA levels in tomato leaves carrying TRV2 (CK) or TRV2:ShARPC3 at 0, 1, 3, 6, 12, and 24 hpi, respectively. (b) Relative mRNA transcript levels of PR1B1 (PR1), β−1,3-glucanase (PR2), and chitinase (PR3; SA pathway-specific gene expression markers) in CK and TRV2:ShARPC3 tomato leaves at 24 hpi as determined by quantitative real-time PCR. Error bars represent the variations among three independent replicates. Number of asterisks indicate statistically degree of significance between CK (TRV2) and TRV2: ShARPC3 plants (Student’s t test, *P < .05; **P < .01)