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. 2020 Dec 18;21(Suppl 2):134. doi: 10.1186/s12863-020-00932-5

Table 1.

(a) results of inheritance mode experiments of mutants, (b) linkage analysis of red body (rb), white eye (we) and black pupae (bp) mutants and (c) GSS backcrossing to we and rb alleles in Anastrepha fraterculus sp. 1

Inheritance crosses F1 phenotype F2 phenotypes Total X2 (3:1)
Wild type mutant
rb WT all population WT 302 297 91 88 778 1.65
WT rb 240 188 76 87 591 2.10
we WT 259 245 69 72 645 3.39
WT we 119 108 33 31 291 1.40
bp WT 528 511 190 194 1423 0.16
WT bp 446 439 155 148 1188 2.99
Linkage crosses F1 phenotype F2 phenotypes Total X2 (9:3:3:1)
WT mutant mutant double mutant
we bp all population WT 997 3201 3612 75 1753 14.43
rb bp 1002 3443 3632 82 1791 10.43
rb we 559 1923 1771 61 989 0.63
Backcrossing WT rb we rb we Total
♂ F1
GSS-89 rb we 250 241 181 183 231 211 216 187 1700

Hypothesis 3:1, X20.05, 1 = 3.841

Hypothesis 9:3:3:1, X20.05, df = 3 = 7.82

WT Wild type; mutant1 = we; mutant2 = bp; mutant3 = rb