Fig. 3.

Bo-Orco expression profile in male and female antennae in pre- and post-mating communication. Relative transcription levels were measured based on their age (days after eclosion), sexual maturity, and mating status. The flies in DAY-4, − 7, − 10 and − 12 after eclosion were unmated and flies in DAY-11 correspond to flies after mating. Expression values were normalized using Rpl19 and actin3 as reference genes and shown relatively to male sample DAY-4. Data are presented as means ± Standard Deviation (SD). Small different letters next to the SD bars indicate a significant difference among different male or female samples respectively, in multiple comparisons within each sex group (P < 0.05, one-way ANOVA with Tukey’s test)