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. 2020 Dec 18;21(Suppl 2):140. doi: 10.1186/s12863-020-00937-0

Table 1.

Primers used for qRT-PCR and generating dsRNAs. Primer sequences for RpL19 and actin3 are described in [28]. Lowercase letters correspond to the T7 sequence

Primer Primer sequence (5’> 3′)
For qRT-PCR c57709_g1_Orco_F3 CTTCTTTGGCGAGAGTGTGA
c57709_g1_Orco_R3 GGCATTCCACGGATAGAAAGA
Rpl19_F AACAAACGTGTACTGATGG
Rpl19_R CACGTACTTTATGTCGTCTG
actin3_F CCACCAGAACGTAAATACTC
actin3_R TCTCATTGAGCGTTTAGAAG
For dsRNA synthesis c57709_Orco_1_F taatacgactcactatagggCAATGAGGAGAAGGAACCCA
c57709_Orco_1_R taatacgactcactatagggCAGTGAAGAACTTCGCTCCC
GFP-F taatacgactcactatagggCCGCCAGTGTGCTGGAA
GFP-R taatacgactcactatagggGATATCTGCAGAATTCGCC