Fig. 3.

Quantitative real-time-PCR to determine relative expression of cellularization genes. Three different embryonic stages (0–6 h, 6–12 h and 12–18 h after egg laying), the second instar larvae (L2), pupae and 5 d old males and females were analyzed. The gene expression was normalized to the reference genes TBP and AK, and related to the Dsnullo expression level at 6–12 h embryos. The mean and standard error from three replicate experiments are shown