Fig. 2.

Transgenic NWS lines show early female lethality. Female specific lethality of four double homozygous two-component lines and an All-in-one line (TD1) was studied at each developmental stage from egg to adult. 1000 eggs were placed in larval diet, containing the following tetracycline (Tet) regimens: 200 μg/mL Tet in the larval diet and 10 μg/mL in adult drinking water, no Tet in the larval diet and 10 μg/mL in the drinking water for one or two days, and no Tet in the larval or adult diet. The number of first, second and third instar larvae, pupae and adult male and females were counted. a The wild type J06 strain on diet lacking tetracycline, b DR2–17/EF1–16, c DR2-11A/EF1–16, d DR2–17/EF1-6B, e DR2–21/EF1–16 and f All-in-one or TD1. All experiments were repeated at least three times and the average and standard deviation (error bars) are shown