Skip to main content
. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: Reprod Biol. 2018 Jun 29;18(3):267–273. doi: 10.1016/j.repbio.2018.06.004

Fig. 3.

Fig. 3.

Effects of follistatin treatment on AKT phosphorylation in early bovine embryos produced with sex-sorted semen. Presumptive zygotes produced with sorted or unsorted semen were cultured with or without 10 ng/ml follistatin for 10 h, then subjected to Western blot analysis for pAKT-Thr308, pAKT-Ser473, tAKT and actin (n = 6 replicates/phosphorylation site, n = 20 embryos/group). Data were normalized relative to the abundance of actin and are expressed as mean ± SEM. Values with different superscripts across treatments indicate significant differences (P < 0.05). Representative Western blot images are shown.