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Isolation and identification of RMGCs from rat retinal Müller cells (RRMCs). RRMCs were isolated and cultured from the retina of a 7-day-old newborn SD rat, and RMGCs were verified using (a) H&E staining, (b) GFAP, and (c) GS immunocytochemical staining after two passages. RMGCs among RRMCs were indicated with GFAP and GS positive staining.
