The interaction between HDGF and miR-384. (a) The putative binding sites of HDGF and miR-384 were predicted by TargetScan. Luciferase activity of A172 (b) and SHG44 cells (c) co-transfected with HDGF 3′-UTR WT or HDGF 3′-UTR MUT and miR-384 mimics or miR-NC. HDGF mRNA expression in A172 (d) and SHG44 cells (e) transfected with miR-384 mimics, miR-384 inhibitor, inhibitor-NC, and miR-NC. HDGF protein expression in A172 (f) and SHG44 cells (g) transfected with miR-384 mimics, miR-384 inhibitor, inhibitor-NC, and miR-NC. (h) The correlation between HDGF and miR-384 (R
2 = 0.3021, P < 0.0001). (i) HDGF expression in tumor tissues, compared with normal tissues. *P < 0.05.