Figure A1.

Upregulation of circHIPK3 facilitated cell proliferation, migration, and glycolysis but repressed apoptosis in lung cancer cells. H1299 cells were transfected with Vector or circHIPK3. (a) QRT-PCR assay for the relative expression of circHIPK3 in transfected cells. (b) CCK-8 assay for the cell viability of the transfected cells. (c) Flow cytometry for the apoptosis of the transfected cells. (d) Transwell assay for the migration capacity of the transfected cells. (e–g) Assessment of glucose consumption, lactate production, and the enzyme activity of HK2 in transfected cells. (h) Western blot assay for the protein expression of Ki-67, MMP-9, and HK2 in transfected cells. *P < 0.05.