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. 2020 Dec 17;10:22163. doi: 10.1038/s41598-020-78695-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in the amount of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecular species, as percentage of total mass spectral signal, in peanut anthers in response to heat stress. Lipid species shown were those that passed both the limit of detection (LOD > 0.0005 nmol) and coefficient of variation (CoV < 0.3) cutoffs (see “Materials and methods” for more details). Values shown are least-squares means. Error bars represent standard errors about the least-squares mean of 16 observations (2 years × 2 blocks × 4 replications) except for Sugg and SPT 06-07, which have 8 observations (1 year × 2 blocks × 4 replications). Least-squares means with different letters are significantly different according to Fisher’s least significant difference (LSD) test at α = 0.05. An asterisk (*) above bars indicate a significant difference at α = 0.05 between AT and HT for that genotype. AT, ambient temperature (31/22 °C for 2018 and 28/22 °C for 2019; average day/night temperatures during the 17-days and 18-days treatment periods in 2018 and 2019, respectively); HT, high temperature (41/27 °C for 2018 and 38/26 °C for 2019). Lipid molecular species are identified as total acyl carbons: total double bonds. Breaks on the y axis indicate a change in scale. Data from 2 years (2018 and 2019) were pooled together for analysis.