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. 2020 Dec 17;10:22163. doi: 10.1038/s41598-020-78695-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Partial least squares-discriminant analysis (PLS-DA) scores plot (a) demonstrating the differentiation of the two treatments: ambient temperature (AT, 31/22 °C for 2018 and 28/22 °C for 2019; average day/night temperatures during the 17-days and 18-days treatment periods in 2018 and 2019, respectively) and high temperature (HT, 41/27 °C for 2018 and 38/26 °C for 2019) and PLS-DA variable importance in projection (VIP) scores (b) that identified the top 15 lipid species that differentiated the treatments. A total of 89 lipid species were analyzed under the two treatments. Data from 2 years (2018 and 2019) were pooled together for analysis. PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; TAG, triacylglycerol. Lipid molecular species are identified as total acyl carbons: total double bonds. Acyl-chain composition is included after each TAG lipid species but does not indicate sn positions.