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. 2020 Dec 17;10:22163. doi: 10.1038/s41598-020-78695-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Dendrogram demonstrating co-occurring lipid groups in peanut anther. All 89 lipid species were clustered using a single-linkage hierarchical algorithm based on Spearman’s correlation coefficient, ρ. Lipid groups with ρ ≥ 0.90 are indicated by blue-colored bars on the dendrogram. The arrows on the dendrogram represent statistically significant changes in the level of each lipid (based on percentage of total signal) under heat stress (41/27 °C for 2018 and 38/26 °C for 2019; average day/night temperatures during the 17-days and 18-days treatment periods in 2018 and 2019, respectively) compared to ambient temperature (31/22 °C for 2018 and 28/22 °C for 2019). Green-colored upward arrows indicate significant increases and red-colored downward arrows indicate significant decreases in the levels of lipids. Data from 2 years (2018 and 2019) were pooled together for analysis. DAG, diacylglycerols; MGDG, monogalactosyldiacylglycerol; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; SQDG, sulfoquinovosyldiacylglycerol; TAG, triacylglycerol. Lipid molecular species are identified as total acyl carbons: total double bonds. Acyl-chain composition is included after each DAG and TAG lipid species but does not indicate sn positions. CLUSTER 3.0 was used to determine clusters (Version 1.59, http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm).