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. 2020 Dec 17;10:22200. doi: 10.1038/s41598-020-79357-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The SDS-PAGE analysis and far-UV CD scans of 3CLpro. (a) SDS-PAGE analysis of the 3CLpro with Coomassie-stain for visualization of the protein bands. The molecular weight marker and 3CLpro sample are loaded to lanes 1 and 2, respectively. (b) Far UV-CD spectra (280–200 nm) of 3CLpro at pH 7.5 for native (green) and denature (black) states collected at 25 °C and after 30 min incubation at 80 °C, respectively. Each spectrum is an average of five CD scans. (c) Far UV-CD spectra of native-state of 3CLpro collected at 25 °C in different pH values of 5.0 (red), 7.5 (green), and 10.0 (blue).