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. 2020 Dec 17;10:22171. doi: 10.1038/s41598-020-79015-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The manufacturing steps of the master mold: (a) the exploded view of the master mold that consists of one 3D printed mold and two CNC micro-milling parts. (b) The assembled master mold. (c) The PDMS casting process. (d) The final PDMS microfluidic chip. [This figure was created by SOLIDWORKS 2016 (www.solidworks.com)].