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. 2020 Dec 17;10:22171. doi: 10.1038/s41598-020-79015-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Steps to produce the master mold and casting with PDMS. (a, b) Two CNC milled parts are bonded to each other permanently using a solvent bonding method. (c) The 3D printed mold is assembled on the CNC mold and fixed with reusable putty adhesive. (d) The cover and microwell PDMS layers bonded together using the plasma bonding method to produce the microwell chip. (e, f) The optical micrographs of PDMS layers with the variable and constant depth (300 µm) microwells.