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. 2020 Dec 17;10:22171. doi: 10.1038/s41598-020-79015-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a–c) The formation of a spheroid in microwells with a depth of 300 μm after 6 h, 48 h, and 96 h. In all microwells, the spheroid is formed. (d) Increment of the compactness of the spheroid for four days in a single microwell. (c) The diameter of the cultured spheroids for each group versus time. There is no significant difference in the diameter (ns) of the spheroid between these five groups of microwells.