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. 2020 Dec 17;10:22228. doi: 10.1038/s41598-020-79285-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Three different intervals of H19 expression accordingly with three classes of cancer degeneration. H19 fold change expression levels in benign biopsies were the lowest (7.6 ± 2.85) followed by atypia and in situ lesions H19 fold change levels, while malignant lesions showed the highest H19 fold change expression (12.3 ± 5.5). Expression levels were normalized to GAPDH expression. Results were expressed as means ± SEM. ANOVA and t-test, *p < 0.05, **p < 0.01, ns p > 0.5.